rohimah mohamud

Researcher | STEM Advocate

Hi, I'm Dr. Rohimah — a passionate researcher, immunologist, educator, and STEM advocate. I love sharing knowledge, exploring science, and helping others grow through curiosity, innovation, and meaningful learning.On this website, you’ll find insights into my journey as an educator and researcher, updates from my involvement in academic conferences and scientific communities, stories and reflections from my work with postgraduate students and outreach in STEM as well as the practical resources for students and lecturers in For You section.Whether you’re here to learn, get inspired, or find tools to support your academic journey, my aim is to make this space a hub for motivation, guidance, and actionable resources.Let’s pursue success with purpose and passion!

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© 2025 Rohimah Mohamud. All rights reserved.

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• 2025 project

research

My research focuses on tumor necrosis factor receptor 2 (TNFR2) as a promising immunotherapeutic target in cancer and inflammatory diseases such as allergy and tuberculosis. With over 15 years in this niche, I lead translational studies using human samples, animal models, and 3D cell cultures to understand TNFR2’s role in immunoregulation.I actively explore natural compounds like vitamin C and honey as modulators of immune response, with vitamin C demonstrating potential as a natural immunotherapy in 3D breast cancer models, and honey formulated into alginate-honey nanoparticles, now filed as a patent for targeted therapy. My full resume is here.My ultimate aim is to translate fundamental discoveries into precision medicine applications, combining nanotechnology, biologics, and next-generation aptamer tools for theranostic (diagnostic + therapeutic) use.To date, my work has led to:More than 1,600 citations, h-index 27Over 20 postgraduates supervised (MSc & PhD)One patent filedNumerous high-impact publications and awards

ANTICANCER, ANTIBACTERIAL AND WOUND HEALING ACTIVITIES OF Nanohoney

This study was conducted by Dr. Mohammad Ahmed Issa Alhatamleh for his PhD project. The aims of his study are to fabricate alginate nanoparticles (ALG-NPs) loaded with stingless bee honey (SBH), and to assess the ability of the honey-loaded ALG-NPs (HALG-NPs) to modulate immune responses, suppress cancer proliferation anmigration, accelerate wound healing and inhibit bacterial growth compared to crude SBH and free ALG-NPs.He concluded that wounds treated with the H-ALG-NPs showed superior healing activity than crude honey. The H-ALG-NPs exhibited 4-fold stronger antibacterial activities than crude honey against the Gram-positive and Gram-negative bacteria. In conclusion, the results suggested that the H-ALG-NPs have enhanced antioxidant, immunomodulatory, anticancer, antibacterial and wound healing activities. This study indicates that the H-ALG-NPs can be fabricated simply by a cost-effective technique with promising therapeutic properties. He completed this study in 2023.

Dr. Mohammad Ahmed Issa Alhatamleh is now a PostDoc at the University of Pittsburgh, USA. His PhD thesis title was Synthesis, Characterization and Evaluation of Immunomodulatory, Anticancer, Antibacterial and Wound Healing Activities of Alginate Nanoparticles Loaded with Heterotrigona itama Honey. More info: Google Scholar Orcid Scopus

Figure 4.34 Representative photographs of the wound areas of the various mice groups. These groups are the H-ALG-NPs, crude honey, the free ALG-NPs, negative control (no treatment) and positive control (MEBO-S). The images taken on day 1, 3, 5, 7, 9 and 11 after injury. (Dr. Mohammad Ahmed Issa Alhatamleh, PhD Thesis, 2023).

role of tnfr2 in tamoxifen-induced breast cancer animal model

This study was conducted by Ms. Ros Akmal Idris for her MSc project. She concluded that TNFR2 was not detected in any group, suggesting it does not play a role or trigger any signalling pathways in this breast cancer rat model. Hence, the findings show that the cancer is more hormone-dependent and less affected by inflammation or immune responses in this model. This is supported by the drop in estrogen (ER) and progesterone (PR) levels after Tamoxifen treatment, and the lack of HER2 expression.Tamoxifen, a selective estrogen receptor modulator (SERM), was an appropriate treatment for this hormone-driven model. Additionally, the balance between pro-apoptotic (Bax) and anti-apoptotic (Bcl-2) proteins seems to be important for tumor cell survival. Tamoxifen influenced both proteins, but its effect on reducing Bcl-2 was more significant."Through this project, I gained valuable lab experience in immunohistochemistry, tissue sectioning, and biomarker analysis. I learned how to assess hormone receptors (ER, PR, HER2) and apoptosis markers (Bax, Bcl-2), which helped me understand how breast cancer responds to treatments like Tamoxifen in a hormone-dependent model.This research also improved my critical thinking and data interpretation skills. Working with animal models taught me the importance of ethical practices and consistency. Overall, this project deepened my interest in cancer and immunological research, and I’m thankful for the opportunity to contribute to this area." - Ros Akmal Idris.

Ros Akmal Idris at 8th AMDI-UNAIR 2024. Her MSc thesis title was The Effects of Tamoxifen on TNFR2 in NMU-Induced Breast Cancer Rat Model. More info: LinkedIn Google Scholar.

Removing breast tumor from tamoxifen-induced breast cancer model. (A) Palpable tumor before surgery. (B) Revealing tumor after incision on the breast skin. (Ros Akmal Idris, MSc Thesis, 2024).

role of tnfr2 in 3D breast cancer cell cultures in respond to vitamin c

Mr. Ali Saeed Ali Mussa from Sudan carried out this project in collaboration with Prof. Rosline and myself. His work addressed the challenge of studying the cancer microenvironment, which is complex and difficult to investigate using conventional 2D cell culture systems. To better mimic real tumor conditions, he employed 3D spheroids — a more advanced and physiologically relevant model.As part of the study on the anti-cancer and immuno-modulatory effects of high-dose vitamin C and low-dose chemotherapy on breast cancer cells, Mr. Ali performed multiple optimizations and successfully established the methods for generating and maintaining 3D spheroids at INFORMM. This included refining spheroid size, ensuring reproducibility, and adapting cytotoxicity and immunological assays for 3D systems.I am Ali Saeed Mussa from Sudan, and I came to Malaysia with one goal — to earn my PhD. My research on “The anti-cancer and immuno-modulatory effects of high-dose vitamin C and low-dose chemotherapy on 3D breast cancer cells” allowed me to gain expertise in 3D cell culture, spheroid generation, cytotoxicity assays, microscopy, immunological techniques, and big data analysis.Balancing a young family and research was challenging, but I published in high-impact journals and completed my thesis. This journey taught me resilience, time management, and the value of interdisciplinary skills as I prepare for a career as a lecturer.

Mr. Ali Saeed Ali Mussa at UMBI Conference 2024. His PhD thesis title was The anti-cancer and immuno-modulatory effect of high-dose vitamin C and low-dose chemotherapy on 3D breast cancer cells. More info: LinkedIn and Orcid

Pharmacological doses of Vit-C induce cytotoxicity of MDA-MB-231 and MCF-7 spheroids. MDA-MB-231 and MCF-7 spheroids were generated and treated for 72 h with five pharmacological concentrations of Vitamin C (1, 5, 10, 15, and 20 mM) or vehicle control (ddH₂O). Representative brightfield images were captured to visualize morphological changes. Scale bar = 500 µm; all images acquired at identical resolution. Get full manuscript here.

• List of student
• 2025 project

stem education

As a researcher and STEM advocate, my long-term mission is to bridge the gap between scientific research and community empowerment. I am deeply committed to translating complex health and immunology concepts into knowledge that communities—especially children and parents—can understand and apply. Through active involvement in NGOs and STEM education initiatives, I aim to nurture a generation of children who are not only academically capable but also health-literate, starting with early awareness of how their bodies and immune systems work. This integration of science, education, and outreach supports the vision of building a healthier, smarter society from the ground up. Full report for 2024 is here.This initiative has gained international recognition and was selected as a case study by the International Science Council, featured in the High-Level Political Forum (HLPF) 2025 on the Sustainable Development Goals (SDGs). We update our activities on TikTok and Facebook.

1. Abstrak Sebuah kejayaan

Saya menulis Abstrak Sebuah Kejayaan dengan harapan agar lebih ramai bangkit dan berjaya dalam hidup, berani bermimpi, dan percaya bahawa setiap usaha ada nilainya.

2. CV template

Download this editable, compact CV template inspired by USM’s format — perfect for quick academic or research profiles. It helps academics summarise key achievements clearly, as interviewers often focus on the numbers, not lengthy documents.

3. eBook for grant application

I’ve compiled key tips and important info for applying international research grants into this ebook — based on my own experience securing a grant from Japan. Enjoy reading, and good luck with your grant journey!

4. Thesis writing and daily Pomodoro writing tracker

Already drowning in research but stuck on the writing? Not sure how to write a discussion section? You’re not alone — and you don’t have to do it the hard way.These free, printable worksheets are designed to keep you on track, stay motivated, and build a strong writing habit — whether you’re working on your thesis, a review article, or any big writing project.Click here to get a 40-Day Writing Challenge, 8-Week Thesis Writing Plan and a Daily Pomodoro Writing Tracker. Click here to get the template for discussion, result and abstract write up. Want an editable version? Just email me!